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Fig. 6. Overgrowth phenotype induced by expression of UAS-ft ${Delta}$ ICD in wild-type wing discs. (A) Control ap-gal4 UAS-GFP wing disc showing dorsal region of expression (green). (B) Overgrowth induced in dorsal cells by ap-gal4 UAS-GFP UAS-ft ${Delta}$ ICD. Ventral regions lacking expression in A and B are similar in size. (C,D) Overgrowth induced by hh-gal4 UAS-GFP UAS-ft ${Delta}$ ICD in posterior compartment (green) of wing pouch and prospective hinge region. There is similar expression of the wing pouch Wg target Dll (purple and right panel) in anterior and posterior cells in C. The distal ring of wg-lacZ expression (purple and right panel) is similar in width in cells inside (arrowhead) and just anterior to (arrow) the region of misexpression in D. (E,F) Expression of UAS-ft ${Delta}$ ICD in the posterior of wing imaginal discs using en-gal4 (E) or hh-gal4 (F). Discs were stained using an antiserum generated against the intracellular domain of Ft (Yang et al., 2002). (E) Expression in ft^G-rv/ft^fd discs. The region of misexpression was identified by the stabilization of Ds (green, left panel); the anti-Ft (purple and right panel) did not crossreact with Ft ${Delta}$ ICD. (F) Expression in wild-type discs. Endogenous Ft (purple and right panel) was stabilized in the region of Ft ${Delta}$ ICD expression, identified by the HA tag on Ft ${Delta}$ ICD (green, left panel). (G) Anti-DE-cadherin (purple and right panel) staining after dorsal expression of UAS-GFP (green) and UAS-ft ${Delta}$ ICD using ap-gal4. Shg levels were slightly decreased dorsally.

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