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Figure 8


Fig. 8. Effects of full-length and deleted ds constructs on wing PCP and crossvein spacing. Normal polarity is indicated by blue arrows and abnormal polarity by red arrows. Proximodistal crossvein spacing in wild type (A) is indicated by a horizontal black double arrow, and is compared with abnormal spacing (red double arrow) in E-H. (A) Wild type. (B) ds05142. (C,D) Partial rescue of ds05142 PCP defects, especially in distal wing blade, by tub-gal4 and UAS-ds (C) or UAS-ds{Delta}ICD (D). (E-G) Expression of UAS-ds (E), UAS-ds{Delta}ICD (F) or UAS-ds{Delta}ECD (G) with tub-gal4 did not induce PCP defects, but reduced crossvein spacing. (H) The crossvein spacing defect induced by UAS-ds{Delta}ECD was stronger using en-gal4, but there were no PCP defects at the sharp boundary of misexpression between L3 and L4. (I-L) PCP in the posterior wing. (I) Wild type. (J,K) Expression of UAS-ds (J) or UAS-ds{Delta}ICD (K) in the central region of the wing using sal-gal4 results in the orientation of hairs away from the region of misexpression. (L) sal-gal4 and UAS-ds{Delta}ECD has no effect on PCP.





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