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Fig. 4. Fgf10 and Fgfr2b expression patterns and rescue of the
Fgf10-/- mammary defect by recombinant FGF10.
(A,B) In situ hybridization for Fgf10 on an E10.0 (30
somite) wild-type embryo; whole-mount (A) and a 40 µm vibratome section (B)
along the plane indicated by the line in A. Fgf10 expression is
present in the lateral plate mesoderm of the flank. (C-H) Radiolabeled
in situ hybridization on Hematoxylin counterstained sections shows
Fgf10 expression (red) in the somites (white contours in C,D), and
Fgf2b expression (red) in the surface ectoderm (E-H). The arrowed
brackets in E-H indicate the Wolffian ridge (WR) or flank. The mammary line
(ml) overlying the hypaxial bud of the somite (black contour in H) expresses
elevated levels of Fgfr2b. (I-L) Whole-mount in situ
hybridization for Wnt10b, showing mammary placode formation (arrows)
in a flank of a control (I,K) or Fgf10-/- embryo (J,L),
cultured with an implanted bead (circle) soaked in BSA (I,J) or rFGF10 (K,L).
(M,N) Nuclear Fast Red stained section at the level of the lines
through the flank in L. Boxed area in the insets are magnified; broken black
lines outline the placodes. Abbreviations: LPM, lateral plate mesoderm; nt,
neural tube; s, somite stage; som, somite; WR, Wolffian ridge; ml, mammary
line. Panels A-H are not to scale. Scale bars: 1 mm in I-L; 100 µm in
M,N.
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