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Figure 1


Fig. 1. MIS signaling and dynamic expression of Misr2, Alk2 and Smad8 in male rat urogenital ridges. The mRNAs of Misr2 (A-D), Alk2 (E-H), and SMAD8 (I-L) were detected by in situ hybridization (whole-mount and cryosection) in the urogenital ridges of E14.25 (A), E14.5 (B,E,I), E14.75 (C,F,J) and E15 (D,G,K). (H,L) Cryosections at the at the level of the broken lines in G and K, respectively. (M-O) Expression of phosphorylated SMADs1, 5, 8 (P-SMAD) was detected by whole-mount immunofluorescence analysis in E14.5 (M), E14.75 (N) and E15 (O) urogenital ridges of male rats. Arrows indicate the first detected substantial expressions. Arrowheads indicate expressions in the coelomic epithelium (CE). Cranial is oriented towards the top and Müllerian duct to the right of individual images. M, Müllerian duct; T, testis; W, Wolffian duct. Scale bar: 500 µm for all the whole-mount samples (A-C,E-G,I-K,M-O); 50 µm for all the sections (D,H,L).





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