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Fig. 4. MIS causes migration of Misr2-expressing coelomic epithelial cells to the Müllerian duct mesenchyme. Hematoxylin and Eosin (HE) staining (A), immunofluorescent analysis of vimentin expression (B) of cranial transverse sections from E14.5 female urogenital ridges. (C-N) Dynamic change of Misr2 expression, CM-DiI localization and laminin-stained basement membrane in cultured female urogenital ridges with or without MIS treatment. Female urogenital ridges were harvested at E14.5, unlabeled or labeled with CM-DiI (D,F,I,L,N), directly fixed (C,D), cultured with no treatment for 20 hours (E-G) or 40 hours (M,N), or treated with MIS for 20 hours (H-J) or 40 hours (K,L). Arrows indicate the coelomic epithelium (CE). White arrowheads (A,B,J) indicate the basement membrane that separates the coelomic epithelium and subjacent mesenchyme. Arrowheads in H-L indicate the extension of Misr2 expression (H,K), CM-DiI detection in the mesenchyme (I,L) and disruption of the basement membrane (J) under the influence of MIS. M, Müllerian duct; W, Wolffian duct. Scale bar: 50 µm for all the sections.

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