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Figure 7


Fig. 7. Smad5 RNAi enhances and Smad8 RNAi inhibits Müllerian duct regression. E14.5 male rat urogenital ridges were treated with control-siRNA (A,C,E), Smad5-siRNA (B,D) or Smad8-siRNA (F) for 12 hours, and subsequently cultured for indicated periods. Whole-mount in situ hybridization was performed to detect Wnt7a expression. Arrowheads or arrows indicate the disappearing and remaining Wnt7a expression, respectively. Cranial is oriented towards the top and Müllerian duct towards the right of individual images. Scale bar: 500 µm.





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