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Fig. 2. Comparison of H3-K9 acetylation, H3-K4 methylation and H3-K27
trimethylation in Hoxb8 genomic surrounding at 12.5 dpc between
anterior and posterior tissues. (A) Comparative analysis of H3-K9
acetylation at regions 2, 7, 8 and 9. (Left) Whole-cell lysates (WCE) prepared
from anterior and posterior parts of 12.5 dpc embryos were subjected to ChIP
analyses by using anti-acetylated H3-K9. Amounts of immunoprecipitated genomic
DNA (A+ and P+) by anti-acetylated H3-K9 subjected to PCR were equivalent to
that of `Input' DNA loaded in lane 1. Mock-immunoprecipitated DNA (A- and P-)
derived from the same volume of the chromatin fraction as used for
anti-acetylated H3-K9 immunoprecipitation were subjected to the PCR. (Right)
Schematic comparison of H3-K9 acetylation between anterior and posterior
tissues. The relative quantity of each genomic region in immunoprecipitated
genomic DNA from anterior and posterior tissues was estimated by referring to
`Input' DNA isolated from the initial lysates and enrichment values against
the initial lysate are represented by the black and gray bars, respectively.
(B) Comparative analysis of di- and trimethylation of H3-K4 at the
region 2, 7, 8 and 9. Representative results (left) and schematic summary
(right) are shown. (C) Comparative analysis of trimethylation of H3-K27
at the region 2, 3 and 8. Representative results (left) and schematic summary
(right) are shown.
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