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Fig. 2. Effect of tendrils mutations on terminal cell branching.
(A) Fluorescent images of homozygous tendrils+ and
tendrils- DB terminal cells clones in sixth tracheal
metamere of wandering third instar larvae. White boxes, region enlarged in
bright-field image below showing lumen. Scale bars: 20 µm in cytoplasm; 10
µm in lumen. (B) Quantification of terminal branching phenotype of
tendrils alleles. Number of terminal branches in DB terminal cell
clone was counted and compared with number in control contralateral
tendrils+ terminal cell. Values shown are mean
(±s.e.m.). n, number of terminal cells analyzed. (C)
Quantification of lumen phenotype of tendrils alleles. The number of
lumens in each clone was counted and divided by the number of terminal
branches. Values above six lumens per branch are plotted together because of
difficulty in obtaining accurate counts at high lumen density. The severity of
terminal branch loss (B) parallels the severity of the lumen phenotype (C) in
this series of alleles.
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