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Figure 4


Fig. 4. Ablation of Fgf8 in crescent mesoderm increases apoptosis and decreases proliferation in foregut endoderm and AHF mesoderm. Transverse cryosectioned Fgf8;MesP1Cre mutants/controls stained with anti-PHH3 (green, mitotic cells) and Hoechst (nuclei), and for apoptosis (TUNEL, red). Sections proceed anterior (top) to posterior (bottom), indicated by the white arrow. (A,B) 0ss; abundant proliferation and minimal apoptosis are detected in control mesoderm (M), endoderm (E) and neuroectoderm (NE) (A, green arrowheads). The number of proliferating cells in the mutant (B) mesoderm appears to be decreased. (C,D) 2ss; increased apoptosis in mutant (D) endoderm (white arrowheads). (E,F) 4ss; increased apoptosis in mutant endoderm (white arrowheads). Many cells are proliferating in the control heart tube mesoderm (HT, white box, green arrowheads) but only a few in the mutant (red arrowheads). (G-J) 9ss; excess apoptosis in the midline endoderm (white arrowheads) and developing OFT of mutants (H,J, red arrows; see also Fig. 3N'',P''). More proliferating cells are detected in the pharyngeal epithelia (green arrowheads; EC, ectoderm; E, endoderm), SM (yellow arrowheads) and contiguous OFT (yellow arrows) of control. Posterior sections in panels I and J are at twice the magnification of those in G and H. pa1, pharyngeal arch 1.





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