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Fig. 4. Tissue-specific ablation studies using Nkx2.5^Cre and TnT-Cre lines. Fgf8^lacZ expression at E8.5 (A,A') overlaps with expression of Nkx2.5^Cre in the OT, SM and PE (B,B'). Additionally, Nkx2.5^Cre and Fgf8^lacZ expression coincides in the CM at E9.5 (D-D'' compared with E-E''). TnT-Cre expression overlaps with Fgf8^lacZ only in the OT myocardium (C,C',F-F''). Noted domains of Nkx2.5^Cre recombination that coincide with Fgf8^lacZ include the OT, SM, CM and lateral PE, but not surface ectoderm [except for first arch (D',D'',E',E'' and data not shown)]. In situ hybridization analysis for Fgf8 was performed at E8.5 (G,H) and E9.5 (I,J) to confirm genetic ablation in these regions with residual expression in the ectoderm. Fgf8 mRNA is markedly reduced in the mutants (H,J) compared with normal controls (G,I). OT, outflow tract; SM, splanchnic mesoderm; PE, pharyngeal endoderm; A, atria; V, ventricles.

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