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Fig. 5. The anterior heart is truncated in Nkx2.5^Cre/+; Fgf8^flox/- mutants. Analysis of normal (A,C,E,G,I) and mutant (B,D,F,H,J) embryos at E9.5 was performed by whole mount in situ hybridization (A-H) or lacZ stain (I,J) for the genes as indicated. Mhc is expressed in the myocardium. Wnt11 is normally expressed in the OT (C, black arrowhead), but is not expressed in mutants (D, white arrowhead). The boundary of Anf expression in mutants (E,F, black arrowhead) is in close proximity to the aortic sac, indicating virtual absence of OT/RV. Mlc2v-negative myocardium of the proximal OT (G, black bracket) is absent in the mutant (H, white arrowhead). Bmp4^lacZ-expressing cells are essentially absent in the OT (J, white arrowhead) and splanchnic mesoderm (J, white bracket) compared with normal controls (I, black brackets). 1, first pharyngeal arch; OT, outflow tract; LV, left ventricle; RV, right ventricle; A, atrium.

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