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Fig. 5. Anti-BDNF decreases synapse density per dendrite branch. (A)
Reconstructions of a sample tectal neuron imaged at 0, 4 and 24 hours further
illustrate the onset of anti-BDNF effects on postsynaptic specialization
density and dendritic arbor morphology. Scale bar: 20 µm.
(B,C) High-magnification images of individual dendritic branches
in (B) control and (C) anti-BDNF treated tadpoles compare changes in PSD95-GFP
puncta per branch, over the 48 hour imaging period. Scale bar: 5 µm.
(D) A measure of the effects of anti-BDNF on postsynaptic
specialization density per branch was obtained by counting the number of
PSD95-GFP puncta at each observation interval in branches with lifetimes of
more than 48 hours (that were present at all observation time points, from 0
to 48 hours). Data were normalized for the change in branch length and are
presented as the percent of its initial value at the time of treatment
compared with control. Bars indicate s.e.m. n=25 branches in control
and n=25 in anti-BDNF. *P
0.05,
**P 0.005 compared with control.
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