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Fig. 1. Substrate-bound gradients fabricated by microcontact printing.
(A) Schematic of a growth cone in a continuous gradient.
(B,C) Growth cones in different discontinuous gradients
fabricated by microcontact printing: (B) a steep gradient built by an array of
stripes and (C) a shallow gradient built by dots. Antibody-stained ephrin is
shown in red; phalloidin-stained actin in black. (D,E) The
gradients shown in B and C in lower magnification extend over a distance of
250 µm. The relative slope 1 of the gradient in D is reduced to 1/3 in E by
interrupting the ephrin stripes. (F) Averaged fluorescence intensities
measured in a 20-µm-wide measuring field moved along the axis of the
gradients in D (blue curve) and E (red curve). Scale bars: 15 µm in B,C; 50
µm in D,E.
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