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Fig. 2. Temporal, but not nasal, RGC axons form stop zones in ephrin
gradients. (A) Nasal growth cones overgrow an ephrin gradient
without being affected. (B) By contrast, temporal growth cones form a
distinct stop zone in the ephrin gradient. As new axons continuously leave the
explant, growth cones are observed in the area between the explant and the
stop zone. Arrows mark growth cones that do not respond to the gradient. To
the right, the ephrin gradient is shown in black with a red arrowhead marking
the beginning of the gradient. Antibody-stained ephrin is shown in red;
phalloidin-stained actin in black. Scale bar: 100 µm.
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