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Fig. 4. The position of the stop zone depends on the slope of the gradient.
(A) Temporal axons in three different gradients printed with 8 µg/ml
ephrinA5. Antibody-stained ephrin is shown in red; axonal actin in black. In
the steepest gradient (relative slope 1) the stop zone is located at the
beginning of the gradient. In shallower gradients (relative slopes 2/3 and
1/3), stop zones are shifted deeper into the gradient. The position of the
stop zone is marked with arrowheads and the overgrown distance within the
gradient is indicated in µm. The axis to the right (in µm) corresponds
to the x-axis in B. Scale bar: 100 µm. (B) Standardized fluorescence
intensity curves of ephrin and the corresponding axonal fluorescence are
depicted in blue (gradient slope 1), light blue (2/3) and red (1/3). The
discontinuity of the gradient reflects in the sinuous course of the ephrin
fluorescence intensity curve. The x-coordinate of the intersection of each
axonal curve with the arithmetic mean (pink line) is defined as the position
of the stop zone. The shallower the gradient, the further the stop zone is
shifted in the direction of the positive x-axis.
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