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Figure 4


Fig. 4. The egg cylinder of Nodal mutants does not reach the size that is required for DVE formation. (A) In normal embryos, DVE formation monitored by HexP-GFP expression occurs only when the apex of the egg cylinder has reached a certain distance from the ExE (epiblast length). (B) While the length of the epiblast is difficult to measure in Nodal mutants due to the lack of a sharp epiblast/ExE boundary, a reduction in overall size (conceptus length) is readily apparent. Note that Nodal mutants at E5.5 do not reach the conceptus length that appears to be required for the DVE to be induced.





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