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Fig. 2. Identification of quail ECs and HCs in chick CAM after parabiosis.
(A-C) Sambucus nigra lectin (SAMB)/QH1 double staining. (A)
The endothelium (arrow) of a chick vessel (v) stained by SAMB. Scale bar: 25
µm. (B,C) One isolated interstitial quail
SAMB+/QH1+EC. Scale bar: 15 µm. (D,E)
QH1/VEGFR2 double staining: a QH1+EC expresses VEGFR2 transcripts
(arrow); a QH1+HC does not (arrowhead). Scale bar: 25 µm.
(F-H) LEP/QH1 double staining: an elongated
LEP-/QH1+EC (white arrow) surrounded by chick
(LEP+/QH1-, white arrowheads) and quail
(LEP+/QH1+, blue arrowheads) macrophages. Scale bar: 25
µm. (I-L) LEA/QH1 double staining: (I-K) two quail
LEA-/QH1+ECs (white arrows) present in the vicinity of a
chick LEA+/QH1- macrophage (white arrowhead) and a round
quail LEA-/QH1+HC (blue arrow). Scale bar: 25 µm. (L)
One quail (LEA+/QH1+, blue arrowhead) and one chick
(LEA+/QH1-, white arrowhead) macrophage are present.
Scale bar: 15 µm. (M-O) GRL2/QH1 double staining: an elongated
GRL2-/QH1+EC (white arrow) identified among chick
(GRL2+/QH1-, white arrowheads) and quail
(GRL2+/QH1+, blue arrowhead) hematopoietic precursors,
and a quail GRL2-/QH1+ macrophage (blue arrow). Scale
bar: 10 µm. (P-T) QH1+HC distribution. QH1+HCs
are found in muscle as extravasated cells (P, arrowheads) or in the bone
marrow as osteoclasts (Q, arrowheads). Scale bar: 80 µm. BM, bone marrow;
ca, cartilage; mu, muscle. (R-T) QH1/
SMA double staining shows that
QH1+HCs never reach vessel walls. *, vessel lumen. Scale
bar: 70 µm.
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