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Figure 3


Fig. 3. Behavior of CECs/EPCs. (A-I) QH1+EC distribution. (A) A whole section of a capillary is quail-derived. Scale bar: 50 µm. (B) Chimeric capillary (*) with a QH1+EC (brown) and two chick QH1-ECs (arrowheads). Scale bar: 20 µm. (C) A larger vein (*) with one QH1+EC (arrow). Scale bar: 45 µm. (D) Among lymphatic vessels (*) surrounding a CAM artery (A), one is chimeric (arrow). Scale bar: 90 µm. (E) A QH1+EC (arrow) in the bone marrow. Scale bar: 45 µm. (F-I) Confocal images of whole-mount SAMB/QH1 double staining on chick CAM. (F) A quail SAMB+/QH1+EC (*) integrated in the superficial chick SAMB+/QH1- vascular plexus. Scale bar: 10 µm. (G,H) A SAMB+/QH1+ vascular cord in contact with the chick SAMB+ plexus (arrowhead). Scale bar: 15 µm. (I) A chimeric vascular cord with quail (white arrowheads) and chick (blue arrowheads) regions linked to a chick vessel (V) through a chick vascular bridge (arrow). Scale bar: 40 µm. (J-L) Conserved migratory potential of CECs/EPCs after tissue integration. (J) Chick `parabiotic' liver (grL) grafted onto the CAM of a chick host: one QH1+EC (arrow) is present. (K) A QH1+EC (arrow) is present in the mesenchyme of an E3 chick limb bud grafted next to a chick `parabiotic' liver. Scale bar: 80 µm. (L) Two QH1+ECs (arrows) originating from the grafted `parabiotic' liver have reached a sinusoid of the chick host liver (hL). Scale bar: 15 µm.





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