spacer gif spacer gif spacer gif spacer gif spacer gif
QUICK SEARCH:   [advanced]


spacer gif
     Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents    

Right arrow Help viewing high resolution images
Right arrow Return to article
(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.


Figure 4


Fig. 4. Mobilization of CECs/EPCs in parabiosis (part 1). (A) An E13 chick wing immediately after a wound: the limb is on the CAM (*) and the size of the wound is delimited by two hemorrhagic dots (arrowheads). (B) The wound (arrowhead) 1 day later, after the shortening of feather buds. Scale bar: 5 mm. (C) Transverse section of an E14 wounded wing showing elongated QH1+ECs (arrows) in the interstitium and integrated in a vessel (*) as well as round QH1+ HCs (arrowheads). In a contralateral E14 wing (D), QH1+ECs (arrow) and QH1+HCs (arrowheads) are less numerous. Scale bar: 100 µm. (E,F) Sections of chick CAM from parabiosis 1 day after wounding. (E) After wounding of veins, there are numerous QH1+ECs (arrows) and QH1+HCs (arrowheads). (F) After wounding of arteries, QH1+ECs (arrows) and HCs (arrowheads) are more rare; *, vessel. Scale bar: 40 µm. (G,H) QH1/TUNEL double staining illustrating that QH1+CECs/EPCs (arrow in G) and QH1+HCs (arrowhead in G) are TUNEL-, while apoptotic nuclei are present in the CAM ectoderm (arrowheads in H). Scale bar: 35 µm. (I) Chick wing (W) 6 days after grafting onto the CAM of a `parabiotic' chick embryo. (J) Chick lung (L) 9 days after grafting onto the CAM of a `parabiotic' chick embryo. Scale bar: 3 mm. (K) Transverse section of a grafted limb showing QH1+ECs (arrows) and QH1+HCs (arrowheads) around the cartilage (ca). (L) transverse section of a grafted lung with a QH1+EC (arrow) integrated in a vessel endothelium (*) and QH1+HCs (arrowheads) in the mesenchyme surrounding a parabronchius (p). Scale bar: 100 µm. (M-O) BrdU incorporation in an E15 parabiosis 9 days after grafting of a chick limb bud onto the chick CAM. (M) In a feather bud of the grafted limb, numerous nuclei are BrdU+ (brown dots); however, QH1+ECs (arrows) present in the pulp are BrdU-. Scale bar: 70 µm. (N) In the corresponding `parabiotic' host chick heart, BrdU is incorporated into the myocardium (brown dots), but QH1+ECs (arrows) and QH1+HCs (arrowhead) are BrdU-. Scale bar: 35 µm. (O) A double stained QH1+/BrdU+EC in a myocardiac capillary (c), the brown nucleus (*) is surrounded by the dark blue QH1 staining. Scale bar: 15 µm.





Right arrow Return to article