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Figure 5


Fig. 5. Quantification of the QH1+ cell mobilization. (A) Kinetics of the wound healing process: a specific, rapid and significant increase in the number of QH1+ECs in wounded wings in comparison with control limbs or with contralateral wings is observed. The specificity of this mobilization is attested by the similar number of QH1+ECs found in livers and hearts isolated from control or wounded chick embryos. (B) During the wound healing process, the QH1+HC number did not vary significantly between control, contralateral and wounded wings. (C) Wounds on CAMs lead to a significant increase in the QH1+EC number when veins are injured compared with control CAM or wounded arteries. (D) In grafting experiments on CAM, the QH1+EC number is significantly greater in the grafted limb buds than in the viscera. (E-G) VEGF-treated CAM assays: while the number of vessel branches is significantly increased in VEGF-treated CAMs (E), by comparison with PBS-treated CAMs (E), the overall QH1+EC number (F) and the number of QH1+ECs per branch (G) do not vary significantly between treated and control CAMs.





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