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Figure 6


Fig. 6. Mobilization of CECs/EPCs in parabiosis (part 2). (A) An endothelin 1-producing CHO nodule (No), 5 days after cellular implantation onto a `parabiotic' chick CAM. Capillaries are organized in a spoke-wheel formation (*) converging to the nodule. Scale bar: 1 mm. (B) The nodule (No) is wrapped in the CAM, and QH1+ECs (arrows) participate in its vascularization. Scale bar: 160 µm. (C) Higher magnification of QH1+ECs invading the nodule (arrows): one cell is in a capillary endothelium (*), two are located interstitially. Scale bar: 20 µm. (D-G) `Parabiotic' chick CAM 4 days after VEGF (D) or PBS (E) treatment: increased vessel branching (*) is apparent in D compared with E. Scale bar: 1.5 mm. (F,G) The vessel density (*) is higher in VEGF-treated (F) than in PBS-treated (G) CAM. Although the inflammatory response leads to an invasion of a great number of QH1+HCs (brown dots) in both treatments, the number of QH1+ECs (arrows) is similar. Scale bar: 90 µm. (H,I) Mobilization in absence of bone marrow: wounds on `parabiotic' chick wings (H) and grafts of chick limb buds onto `parabiotic' chick CAM (I) lead to an invasion of QH1+ECs (arrows) and QH1+HCs (arrowheads). ca, cartilage. Scale bars: 90 µm in H; 70 µm in I.





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