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Fig. 8. Dachs protein localization. High magnifications of wing imaginal discs, stained for Fat (red, C-F) or E-cadherin (red, A,B,G) and Dachs:V5 (green), with GFP expression in blue. For experiments depicted in A and B, discs were treated in parallel, and images of clones were captured with identical confocal settings. (A) Dachs:V5-expressing clone in AyGal4 UAS-dachs:V5[9F] UAS-GFP. Arrows indicate distal clone edges with higher Dachs:V5; arrowheads indicate proximal clone edges with lower Dachs:V5. (B) Fat and Dachs:V5-expressing clone in AyGal4 UAS-fat UAS-dachs:V5[9F] UAS-GFP. (C) Fj and Dachs:V5-expressing clone in AyGal4 UAS-dachs:V5[9F] UAS-fj[6a2] UAS-GFP. Arrowhead indicates elevation of Fat, and coincident absence of Dachs:V5, at the edge of the clone. (D) Fj-expressing clone in AyGal4UAS-fj[6a2] UAS-GFP. Arrowhead indicates elevation of Fat at the edge of the clone. (E) Ds-expressing clone in AyGal4 GS-ds UAS-GFP. Arrow indicates elevation of Fat at the edge of the clone. (F) Ds and Dachs:V5-expressing clone in AyGal4 UAS-dachs:V5[9F] GS-ds UAS-GFP. Arrow indicates elevation of Fat, and coincident elevation of Dachs:V5, at the edge of the clone. (G) fat mutant clone (arrow), marked by absence of GFP, in tub-Gal4 UAS-dachs:V5[18-2]. Dachs staining is slightly elevated within the clone. (H) Vertical section through the apical-most 4.5 µm of the clone depicted in A. Most Dachs:V5 staining overlaps E-cadherin at the apical junctions, but there are discrete puncta (arrow), as well as more diffuse staining, in the cytoplasm. (I) Western blot, with anti-V5 or ant-Actin (control) on discs from: (1) fat⁸/fat^G-rv; tub-Gal4/UAS-dachs:V5[18-2], (2) fat⁸/+; tub-Gal4/UAS-dachs:V5[18-2] and (3) wild type.

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