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Fig. 1. Inhibition of FGF2-dependent NPC maintenance by an inhibitor of
JAK2. (A) Neuroepithelial cells prepared from E12.5 mouse neocortex
were dissociated and cultured in suspension for 7 days with FGF2 (20 ng/ml) in
the absence or presence of either the MEK inhibitor U0126 (10 µmol/l), the
PI3K inhibitor LY294002 (20 µmol/l) or the JAK2 inhibitor AG490 (10
µmol/l). The number of generated neurospheres was then counted.
*P<0.01. (B) Neuroepithelial cells prepared from
E12.5 mouse neocortex were dissociated and cultured in suspension with FGF2
(20 ng/ml) for 2 days and in the additional absence or presence of U0126,
LY294002 or AG490 for 1 day. The resulting primary neurospheres were
dissociated and cultured in suspension for 7 days with FGF2 (20 ng/ml) and EGF
(20 ng/ml) in the absence of inhibitors. The number of secondary neurospheres
was then counted. *P<0.01.
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