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Fig. 6. Regulation of DLL1 expression by STAT3. (A-D) Brain sections
of E16.5 mice were immunostained with anti-STAT3 and anti-DLL1, as indicated.
DLL1 was detected in STAT3-expressing cells. Dorsal side is up in all panels.
Scale bars: 400 µm in A; 80 µm in B. (E) NPC cultures prepared
from the neocortex of E12.5 STAT3flox/flox mice were infected with
retroviruses encoding GFP alone (Control) or both GFP and Cre-recombinase
(Cre). After culture for 2 days, the cells were subjected to RT-PCR analysis
for the indicated mRNAs. Data are expressed relative to the corresponding
normalized value for control cells. *P<0.01. (F)
NPC cultures infected and cultured for 2 days as in E were subjected to
immunoblot analysis with the indicated antibodies. (G) Promoter regions
of the mouse DLL1 genes that contain consensus sequences for STAT3 binding
(white arrowheads and the nucleotides in boldface type). NPC cultures prepared
from the neocortex of E12.5 mice were subjected to ChIP analysis with
anti-STAT3 or control immunoglobulin G (IgG). The immunoprecipitates were
analyzed by PCR with primers spanning the indicated regions (underlined) of
mouse Dll1.
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