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Fig. 3. Expression pattern of nvd-Dm. (A-C) In situ
embryonic expression (see also Fig. S4 in the supplementary material).
nvd-Dm mRNA was not detected during early stages of embryogenesis,
such as preblastoderm stage (A) or stage 8 (B). At stage 17 (C),
nvd-Dm expression was detected specifically in the ring gland
(arrowhead). (D) Brain-ventral nerve cord-ring gland complex of
wandering stage of third instar larva. (D') Higher magnification
of D. Expression was detected only in the region of PG cells, but not in
either the corpus allatum (CA) or the corpus cardiacum (CC). (E)
Quantitative RT-PCR analysis of nvd-Dm transcript in several larval
tissues from wandering third instar larvae. nvd-Dm/rp49 indicates the
levels of nvd-Dm mRNA normalized to the levels of internal
rp49 mRNA. The normalized nvd-Dm mRNA level in the ring
gland is set as 1. (F) Cyclic expression of nvd-Dm in the ring
glands during the second and third larval stages shown by the quantitative
RT-PCR. The normalized nvd-Dm mRNA level from the wandering third
instar larvae is set as 1 (±s.e.m.; n=3). (G-I) Ovarian
expression. nvd-Dm mRNA was detected in the nurse cells (G,H; arrow),
whereas oocytes was devoid of nvd-Dm expression (H; arrowhead). No
staining was obtained for the sense RNA probes in ovaries (I), embryos and
larvae (data not shown).
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