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Fig. 6. Developmental progression of nvd-Dm RNAi animals by feeding
20-hydroxyecdysone, cholesterol and 7-dehydrocholesterol. nvd-Dm
RNAi (black arrowheads) and control larvae were fed yeast paste supplemented
with either 3.3% ethanol, 1 mg/ml 20-hydroxyecdysone (20E) or 0.5% wet weight
of cholesterol (C) or 7-dehydrocholesterol (7dC). All control animals grew to
the third instar larvae 96 hours AEL. Red arrowheads indicate teeth of the
mouth hooks. (A,B) nvd-Dm RNAi animals fed
20E-containing food during only 30-42 hours after egg laying (AEL) molted to
the second instar larvae. However, 96 hours AEL they showed small body size
(A) and still possessed the second instar-type mouth hook (B).
(C,D) nvd-Dm RNAi animals with 20E 30-42 hours and
54-66 hours AEL molted to the third instar larvae. (E) nvd-Dm
RNAi animals fed 20E 30-42 hours, 54-66 hours and 78 hours AEL. After becoming
third instar larvae, 45% and 5% of the nvd-Dm animals pupariated and
eclosed (E), respectively. (F) nvd-Dm RNAi animals with 7dC.
All the animals grew to adults. (G,H) nvd-Dm RNAi
animals with excessive C had the second instar-type mouth hook 96 hours AEL
(H). Their body size nearly reached the size of control animals (G). Scale
bar: 1.0 mm for A,C,E,F,G; 10 µm for B,D,H. (I) Lethality of animals
in the feeding experiments. The stage of development at which an animal died
is depicted as a percentage of animals that died at that stage. `30-42',
`54-66' and `78-' in brackets refer to the periods (AEL) at which animals were
fed 20E-treated yeast pastes. `n' refers to the total number of animals.
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