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Fig. 1. calderón expression in embryonic and imaginal
tissues. (A) Genomic organization of the cald locus. The
P-element insertions cald-gal4 and EP1072 are shown as blue
triangles. (B) Dorsal view of a cald-Gal4; UAS-lacZ embryo at
stage 13. Note lacZ expression, visualized by histochemical staining
for ß-gal activity, in the amnioserosa (black arrowhead) and in the
central nervous system (CNS, white arrowhead). (C-G) Lateral view
(anterior left, dorsal up) of embryos showing the distribution of
CG13610 transcripts from stage 5 (C) to 13 (G). Early embryos (C)
have uniform levels of cald expression. Gastrula embryos (D) have
higher levels of cald transcripts in the most dorsal cells. From germ
band extended (E,F) to germ band retracted embryos (G). cald
expression is restricted to the amnioserosa and the CNS. (H) Dorsal
view of an embryo at stage 13 showing cald expression in the
amnioserosa and the CNS, which reproduces cald-Gal4 expression
(compare with B). (I,K,M) cald-Gal4:UAS-GFP
staining in wing (I), eye-antenna (K) and leg (M) imaginal discs.
(J,L,N) Wing (J), eye-antenna (L) and leg (N) imaginal
discs showing cald transcripts. cald transcripts reproduce
the cald-Gal4 expression. (O) Alignment of Calderon and the
human protein SLC22A4. Both proteins have 11 transmembrane domains with a
Major Facilitator Superfamily domain (boxed amino acids) and a Sugar
Transporter domain (red amino acids). Identical amino acids are identified by
a star. Double dots label conserved substitutions and single dots
semi-conserved substitutions. The two proteins are 36.4% identical.
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