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Fig. 3. calderón adult phenotype. (A) Graph depicting the developmental delay of cald^R161 animals. The length of embryonic (E, green), larval (L, red) and pupal (P, blue) stages is shown for cald^R161 and wild-type animals. (B) cald^R161 animals are smaller than wild-type ones. (C) cald^R161 and wild-type heads; note the cald^R161 eye is markedly smaller. (D) cald^R161 and wild-type wings. Note the cald^R161 wing is smaller than the wild-type one. (E) Histogram plotting the size and cell density ratio of calderón/wild-type animals. Ratio (fly length): 0.9±0.04; ratio (eye size): 0.8±0.05; ratio (wing size): 0.85±0.04; ratio (cell density): 1.3±0.16. Number of scored flies: n (wt):7; n (cald):9. Number of scored eyes: n (wt):7; n (cald):9. Number of scored wings: n (wt):6; n (cald):6. Number of scored wings for cell density counting: n (wt):7; n (cald):9. (F,G) cald^R107/cald^EP1072 male (F) and female (G) animals are smaller than wild-type ones when raised at 18°C, but nearly wild-type at 25°C.

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