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Fig. 4. calderón mutant cells are eliminated by cell competition. (A-C) Wing discs with cald^R107 mutant clones marked by the absence of expression of the lacZ gene, as visualized with an antibody against the ß-Gal protein (grey). Twins were marked by the presence of two copies of lacZ (white). Larvae were dissected 24 (A), 48 (B) and 72 (C) h after clone induction. Twins were larger than mutant clones and 72 h after induction cald mutant cells disappeared. (D) Rescue of cald^R107 mutant cells by expression of an UAS-cald transgene using the MARCM technique. Clones were visualized 72 h after induction by the expression of an UAS-GFP transgene (white). (E,E') cald^R107 mutant clones marked by the absence of the GFP marker (green) and visualized 24 h after induction. Expression of the activated form of the Caspase-3 protein is shown in red. The upper panel shows an XY confocal section of the wing pouch. The right and lower panels show YZ and XZ sections of the same wing discs at the level of the white lines. (F,F') brinker (brk) expression in cald mutant cells. cald^R107 mutant clones marked by the absence of the GFP marker (green) show an up-regulation of brk-lacZ expression (in red).

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