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Figure 6


Fig. 6. calderón clonal phenotypes. (A,B) cald mutant cells are small in imaginal discs. Minute+ caldR107 mutant clones marked by the absence of the GFP marker (green) in the wing imaginal disc. Cell membranes are delineated by Phaloidin (red in A) and cell nuclei are labelled by DAPI (blue in B). dFOXO (red in B) remains cytoplasmic in cald clones. The white trace outlines the mutant clone in B. (C-F) cald mutant cells are small in the adult. Clones of Minute+ cald mutant cells marked by the absence of the P(yellow+) rescue construct in the notum (C,D), and in the wing margin (E,F). Each hair-like structure is a trichome emanating from a single epithelial cell. Note the reduced size and increased density of cald cells (red arrow) compared with surrounding yellow+ ones (black arrowhead).





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