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Figure 2


Fig. 2. The brat mutation affects larval central brain proliferation in a cell-autonomous manner. Wild-type (A,B) and brat11 (C,D) MARCM clones labelled with CD8::GFP in third instar larval brain hemispheres (A,C) and ventral ganglia (B,D), counterstained with the DNA dye TOTO-3. When induced at low frequency in newly hatched larvae, wild-type clones contain progeny of a single neuroblast occupying a small area of third instar larval brain (single clone shown in A). Similar heat shock conditions generate brat11 mutant clones of large size, which appear difficult to resolve as single neuroblast lineages (two or more merged clones are shown in C). In ventral ganglia, brat11 mutant clones (D) are recovered at similar frequency as in wild-type clones (B) and appear indistinguishable in size and shape. Scale bars, 50 µm. Genotypes: (A,B) hsFLP/+; FRT40A, UAS-mCD8::GFPLL5, UAS-nlslacZ20b/FRT40A, tubP-GAL80LL10; tubP-GAL4LL7/+; (C,D) hsFLP/+; FRT40A, brat11, UAS-mCD8::GFPLL5, UAS-nlslacZ20b/FRT40A, tubP-GAL80LL10; tubP-GAL4LL7/+.





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