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Fig. 7. pros can promote cell cycle exit and differentiation of brat mutant cells. Targeted expression of UAS-brat (A,B) or UAS-pros (C,D) in brat¹¹ mutant clones restores wild type-like features of larval central brain lineages. Clones labelled with CD8::GFP (white) were immunostained in late third instar larvae as indicated for each panel. Outline of clones is indicated with dots when GFP label is omitted for clarity. Clones display a limited number of neuroblast-like cells (asterisks) that specifically stain for Grh (A,B,D) or Mira (C). Mitosis is restricted to large nuclei (C,C'; asterisks) or to smaller, closely associated GMC-like cells (C,C'; arrowhead). Most cells in clones project axons contributing to fibre tracts (A-D; arrows) and show nuclear Elav (A,A') as well as nuclear Pros (B,B',D,D'). Scale bar, 40 µm. Genotypes: (A,B) hsFLP/+; FRT40A, brat¹¹, UAS-mCD8::GFP^LL5, UAS-nlslacZ^20b/FRT40A, tubP-GAL80^LL10; UAS-brat^FL1B/tubP-GAL4^LL7. (C,D) hsFLP/+; FRT40A, brat¹¹, UAS-mCD8::GFP^LL5, UAS-nlslacZ^20b/FRT40A, tubP-GAL80^LL10; UAS-pros^L(wt)9D/tubP-GAL4^LL7.

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