|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 3. Retinoic acid rescues pectoral fin field induction in the absence of
paraxial mesoderm. Whole-mount in situ hybridizations. Anterior is towards
the left, dorsal views. (A-D) Expression of tbx5 in pectoral
fin buds is unchanged in ntl(B), but reduced in spt (C) and
absent in ntl/spt double-mutant embryos (D). (E-M) Application
of 10-8 M RA, beginning at 10 hpf, rescues tbx5 expression
in ntl/spt pectoral fins (arrowheads in I,K); rescue occurs in the
absence of myogenic differentiation, demonstrated by the absence of
myod (J,K) and 
-tropomyosin-1 (tpm1)
expression (L,M) in RA-treated double mutants. Four rescue experiments, each
with 40 embryos, were performed (total numbers of phenotypes: 32 spt,
35 ntl, 11 ntl/spt, 82 siblings without mutant
phenotype).
|
