|
|
|
|
|
|
|
||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | ||||
|
Fig. 2. Moz and Hox2 genes function in CNC to control second-segment skeletal
identity. (A-D,H,L,P,T) Facial skeletons from 4 dpf larvae. (A)
Wild type. (B) moz- control side of H. (C)
hox2-MO animal (control side of D). (D) hox2-MO
animal unilaterally rescued with wild-type CNC. (E,I) Schematics
showing unilateral transplantation of wild-type CNC precursors (red) into
moz- hosts. fli1:GFP labels all CNC green.
Wild-type CNC (red) populate the second segment (2) at 32 hpf
(F,J) and by 4 dpf (G,H,K,L) form
largely normal Hm, Sy, Ch, Op and Br skeletal elements in 8/10
moz- hosts. Inset in F is a digital longitudinal section
through the white line and shows co-localization of the transplant lineage
tracer and the fli1:GFP. Lateral is up. In I-L, a hybrid skeleton was
observed. In this example, M'-like (black line in L) and Ch cartilages were
fused together, with M' consisting of moz- host CNC (white
lines in J and K), and Hm, Sy and Ch cartilages and Op osteocytes consisting
entirely of wild-type donor CNC. (M) Schematic of transplantation of
moz- CNC (red and green) into wild-type hosts. Both donor
and host are fli1:GFP+. (N)
moz- CNC (red) populate the second segment (2) at 36 hpf.
(O,P) At 4 dpf, partial PQ'-like (*) and M'-like
(arrow) cartilages consist of moz- donor CNC (white
arrow), and a Sy-like element (arrowheads) forms primarily from wild-type host
CNC. In 9/10 cases, moz- CNC precursors formed jaw-like
cartilages in a wild-type host. (Q) Schematic of transplantation of
moz-; fli1:GFP CNC (red and green) into b1092
hosts (note that hosts are fli1:GFP-).
moz- CNC populate the first two segments (1,2) at 32 hpf
(R) and by 4 dpf form moz--like M' and Pq'
cartilages (S,T) in 6/9 moz+ hosts. Scale
bars: 50 µm.
|
