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Fig. 3. Moz is not sufficient in the endoderm, surface ectoderm or hindbrain to
rescue moz- skeletal development. Wild-type ectoderm
(A-C), endoderm (D-F) and hindbrain
(G-I) precursors from fli1:GFP animals were
unilaterally transplanted into moz-; fli1:GFP
hosts at shield stage. Confocal images show that transplants (red) contributed
significantly to the surface ectoderm (A), facial endoderm and pouches (arrows
in D), and hindbrain (G) at 36 hpf. Insets in A and D are digital longitudinal
sections through the level of the white lines and show that transplanted
tissue did not express the fli1:GFP CNC marker. Lateral is up.
(B,C,E,F,H,I) Facial skeletons of the first two segments from the transplanted
animals at 4 dpf. Cartilages from the sides receiving transplants (B,E,H) were
indistinguishable from the contralateral control sides (C,F,I). No rescue was
seen in 3 ectoderm, 9 endoderm and 12 brain transplants. Scale bars: 50
µm.
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