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Fig. 6. Moz is required for the growth and chondrification of dorsal
second-segment CNC. (A-C) Progressive frames, as indicated,
from time-lapse confocal recordings of first (1) and second (2) pharyngeal
segment development in wild-type fli1:GFP (A),
moz-; fli1:GFP (B), and moz-;
fli1:GFP/wild-type fli1:GFP mosaic (C) animals. Skeletal
outlines are shown at 84 hpf. (A) A subset of CNC precursors are
labelled with a red dye. In wild type (n=4), dorsal second-segment
CNC (arrowheads) adjacent to p1 endoderm (P) form the anterior part of Hm
cartilage. Dorsal first-segment CNC (brackets) form either no cartilage or
neurocranial cartilage (*). Intermediate first-segment CNC (arrows)
contribute to Pq cartilage. (B) In moz-;
fli1:GFP animals (n=6), intermediate second-segment CNC
contribute to Pq' cartilage (arrows). More dorsal CNC make no cartilage
(brackets). In C, small amounts of wild-type CNC precursors (red) were
transplanted into moz-; fli1:GFP animals.
Wild-type second-segment CNC (arrowheads) adjacent to p1 grow and form a
cartilage nodule in the normally cartilage-free dorsal zone of the
moz- host. (D) The skeletal preparation of this
same animal at 5 dpf. Scale bars: 50 µm. See also Movies 1-3 in the
supplementary material.
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