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Fig. 4. Cell-shape changes of the EVL during epiboly correlate with
msn1-mediated recruitment of actin and myosin 2 in the YSL.
(A-F) Analysis of actin and myosin 2 localization in control and
YSL-morphant embryos. Phalloidin staining of embryos at 50% epiboly (A,B) and
co-staining of 75% epiboly embryos with Phalloidin and an anti-phospho-myosin
light chain 2 antibody (C-F). (G,H) Intensity profiles of
Phalloidin in the YSL in the vicinity of the EVL margin in control (G) and
YSL-morphant embryos (H) at 50%, 60%, and 75% epiboly stages. The intensity of
the Phalloidin signal was plotted along a line perpendicular to the EVL margin
(see Materials and methods). Average plots are shown. Scale bar in F: 25 µm
for A-F.
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