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Fig. 4. B8.12 descendants coalesce into the gonad. (A) A gastrula
embryo with DiI-labeled B7.6 cells (arrowhead). (B) A tailbud embryo
with DiI signals in the B8.11 (arrowhead) and B8.12 (arrow) cells. (C)
A stage 6 juvenile, in which the B7.6 cells were DiI labeled, were stained for
CiVH (green). The arrowhead indicates the primitive gonads. Five DiI-labeled
cells (arrowheads) that were also CiVH positive coalesced into the gonads
(inset). (D) A larva in which B7.6 blastomeres were DiI-labeled and the
distal part of the tail containing the B8.12 descendants was cut off. The DiI
signals remained in the B8.11 cells of the tail (arrowhead). (E) A
stage 5 tail-cut juvenile. The DiI-positive B8.11 cells were present on the
gut wall (arrowhead). Owing to the capture of a faint DiI signal, the
auto-fluorescence from the tail debris was relatively prominent (arrow). No
CiVH-positive cells were detected in this juvenile (data not shown).
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