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Fig. 6. Ci-PEM RNA and CiVH protein show different distributions after
the gastrula stage. Embryos were stained for CiVH protein (magenta) and
for Ci-PEM RNA (green). Nuclei were labeled in blue (J,O).
(A-E) A 32-cell stage embryo; (F-J) a gastrula embryo;
(K-O) a tailbud embryo. (B-E,G-J,L-O) Enlarged views of A,F,K.
(A,B,F,G,K,L) Nomarski optics. Although the CiVH protein was released from the
CAB and diffused into the cytoplasm (H,J, arrows), the Ci-PEM RNA
remained in the anterior-most region in the B7.6 cells at the gastrula stage
(I,J, arrowheads), resulting in its inheritance only by the B8.11 cells at the
tailbud stage (N,O, arrowheads).