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Fig. 8. CiVH protein production in the B8.12 cells depends on the translation of
maternally inherited RNA. (A,A') Untreated mid-tailbud-stage
embryos, showing CiVH signals in the B8.11 (arrowhead) and B8.12 (arrow)
cells. (B,B') AcD-treated embryos. Although the treatment
severely affected embryogenesis, strong CiVH signals were detected in the
presumptive B8.11 (arrowhead) and B8.12 (arrow) cells. (C,C')
Puromycin-treated embryos, in which CiVH signals in the presumptive B8.12
cells were almost undetectable. Owing to the capture of a faint CiVH signals
(C' arrow), background signals in the cortical rim were relatively
prominent. (A'',B'',C'') Histochemical
staining for AP activity under each condition. (D) Expression of
Ci-AP, CiVH and ß-tubulin RNAs, analyzed by RT-PCR. A
severe reduction in the expression of Ci-AP mRNA in the AcD-treated
embryos was confirmed by using two different primer sets (AP-1, AP-2). The
negative controls without reverse transcriptase (RT) are shown in the lanes
(-) to the left of each RT-positive lane (+).
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