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Fig. 2. The nurf-1 locus is predicted to encode multiple proteins.
Each protein shares domains with Drosophila NURF301. Only the isoform
containing the HMGA domain is required for antagonism of the synMuv genes.
(A) Genomic structures of the nurf-1 isoforms. Exons are
indicated by black boxes; 5' and 3' untranslated regions are
indicated by white boxes. The predicted translation initiation and termination
codons and the polyadenylation sites are indicated. The locations of the two
deletion alleles are shown. (B) A representation of the NURF-1 protein
and domain structure. (Top) The Drosophila NURF301 protein. (Bottom)
The predicted protein products of the nurf-1 gene. An alignment of
the predicted functional domains of NURF-1 with those of Drosophila
NURF301 is presented in Fig. S3. (C) Reduction of nurf-1a
function suppresses the synMuv phenotype of lin-15AB mutants. RNAi of
the nurf-1a isoform (pEA147) but not of the nurf-1b, nurf-1c,
nurf-1d, nurf-1e isoforms (pEA30) caused suppression of the
lin-15AB synMuv phenotype. In addition, deletion of the
nurf-1a isoform (n4293) but not of the nurf-1b, nurf-1c,
nurf-1d, nurf-1e isoforms (n4295) caused suppression of the
lin-15AB synMuv phenotype. M+ denotes progeny of
heterozygous mutant hermaphrodites, such progeny might retain maternally
inherited nurf-1a gene products.
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