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Fig. 3. Loss of isw-1 function suppresses multiple cell-fate defects
caused by mutations in class B synMuv genes. (A)
isw-1(n3294) suppresses the ectopic expression of PGL-1 in the soma
of L1 larvae in the class B synMuv mutant lin-15B(n744). Anti-PGL-1
staining is shown in green and nuclei (4,6-diamidino-2-phenylindole (DAPI)
staining) are shown in blue. Scale bars: 10 µm. (B) Loss of
isw-1 function suppresses the Tam phenotype of lin-15B
animals and represses normal transgene expression. The ccIs4251
reporter is a simple repetitive transgene that expresses GFP in the nuclei of
body-wall muscles. From left to right, ccIs4251 was expressed in the
following backgrounds: wild type, isw-1(RNAi), lin-15B(n744) and
isw-1(RNAi); lin-15B(n744). Scale bars:100 µm. A
quantification of the Tam experiments is found in Fig. S4. (C)
isw-1 is required for the RNAi sensitivity of the class B synMuv
mutant lin-15B(n744). After exposure of animals to cel-1
RNAi, the number of arrested L2 larvae was scored in at least three
independent experiments. The average percent of L2 arrested larvae is shown.
Error bars indicate standard deviations. (D) The activity of
isw-1 and nurf-1 are required for the larval-lethal
phenotypes of mep-1(q660) and let-418(n3536) mutants. The
percent of sterile adults present at 25°C reflects the suppression of
larval lethality. Error bars indicate standard deviations.
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