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Fig. 7. Abnormal expression of PC markers in the postnatal Ebf2 null
cerebellum. Non-radioactive in situ hybridization (A-G,J-O)
of postnatal (P3) frontal sections with markers of PCs or PC subsets, as
labeled. (H,I) Immunostaining for EphA4, same postnatal stage.
(A,D,G,J,M) Ebf2 expression in corresponding wt sections. (C,F) Loss
of PCs expressing Ebf1, and Sema3A, respectively, in the
mutant's anterior vermis. Arrows in B,E show corresponding territories in the
wt cerebellum. (I) Changes in the striped pattern of EphA4 protein
expression (asterisks in I, control in H) in the mutant vermis. (L,O) loss of
a large number of PCs is revealed by the expression of Ebf3 (L) and
by the striped marker Sema3A (arrows in O). Corresponding wt sections
are shown in K and N (arrows), respectively. Scale bar: 200 µm for A-F,J-O;
230 µm for G-I.
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