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Fig. 8. Ebf2-lacZ transgene expression in the heterozygote. (A)
Wholemount X-gal staining of the adult cerebellum, posterior, dorsal and
anterior views. I-IX: vermian lobules; l.ans, l.para: ansiform and paramedian
lobules, respectively. In the vermis, transgene expression is absent from the
NZ (e.g. lobule X) and CZ (e.g. lobule VIb), expressed in stripes in the CZ
(e.g. lobule VIII) and in the AZ (e.g. lobule III; arrows: lacZ
negative stripes). (B-H) transverse 40 µm sections double-labeled
for the transgene (blue) and ZII (brown). (B) posterior hemisphere (crus II).
The PC stripes P4a+ - P7+ are labeled [for stripe terminology, see Eisenman
and Hawkes (Eisenman and Hawkes,
1993) and Sillitoe and Hawkes
(Sillitoe and Hawkes, 2002)].
Transgene expression is restricted to P-stripes. Higher magnifications of the
regions indicated are shown in D and E. (C) Posterior vermis. Lobule X and
ventral lobule IX (the NZ) are comprised entirely of ZII+ PCs and there is no
transgene expression. The NZ and PZ interdigitate in dorsal lobule IX and by
lobule VIII a clear alternation of ZII+ (P1+ to P4+) and ZII-stripes is
apparent. Ebf2-lacZ expression is restricted to P-stripes. A higher
magnification view of the outlined area is illustrated in F. (G) Crus I shows
transgene expression in the ZII- PC stripes. (H) Anterior vermis (AZ). ZII is
expressed in an array of narrow stripes, P1+ at the midline, P2+ and P3+
laterally on either side. Transgene expression is in the ZII-immunonegative
stripes. Scale bars: in A, 2 mm for A; in H, 1 mm for B,C,G,H; in F, 500 µm
for D-F.
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