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Fig. 9. Profound alterations in cerebellar topography in the Ebf2 null
cerebellum. (A) posterior, dorsal and anterior views of a
wholemount X-gal stained adult cerebellum. The lobules in the vermis are
labeled (I-X). Expression of the transgene is similar to that in the
heterozygote (see Fig. 8) except for a dramatic narrowing on the anterior
lobes due to PC death (I-V).
(B,B'-H,H') Matched pairs of
immunoperoxidase-stained transverse sections from the +/- (B-G) and -/-
(B'-G') vermis; ZII expression from lobule IX to lobule I; SPHK1a
expression in lobule VIII (H,H'). Posterior lobules: alternating
ZII+/- stripes in the heterozygote (IX, VIII, VII) have been
replaced by uniform ZII+ expression domains (IX, VII) or by intermingled
positive and negative PCs as in lobule VIII, where superimposable changes are
observed with SPHK1a immunostaining (H,H'). In the AZ, alternating ZII+
stripes are preserved in the null (E-G: P1+ and P2+ are labeled in F) but
stripe spacing is reduced due to loss of the ZII-immunonegative P1-stripe. The
alterations in stripe patterning are evident in cerebella wholemount-
immunostained for ZII. (I,J) Posterior hemispheres.
(K,L) Posterior vermis: ZII-immunonegative stripes are absent in
L. (M,N) anterior hemispheres. (O,P) anterior
vermis: ZII-stripes in P are present but shrunk, due to perinatal PC
apoptosis. Scale bars: in A, 2 mm for A; in H', 500 µm for
B,B'-H,H'; in I, 1 mm for I,J,M,N; in L, 1 mm for K,L,O,P.
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