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Fig. 3. The presence of the eye is not required for eye muscle differentiation
and specification. (A) Scheme of eye ablation at HH10; dorsal view,
anterior to the top. The optic placode and the region of the forebrain
delivering the optic cup were removed. Pink: unsegmented head mesoderm.
(B-E) Lateral views of HH19 heads; anterior to the top, dorsal to the
right for unoperated sides (B,D), dorsal to the left for operated sides (C,E).
(B-E) Brown staining: Pax6 antibody staining detecting strong expression in
the telencephalon (t), eye, neural tube (nt)/hindbrain. Note, in operated
embryos the eye is missing; only the rest of the optic stalk remained (C,E,
open arrowhead). (B,C) Lbx1 (blue staining) revealing normal lateral
rectus anlagen. (D,E) Also Myf5 expression (blue) is wild type. Scale
bar: 200 µm. do, dorsal oblique EOM; dr, dorsal rectus EOM; hm, unsegmented
head mesoderm; hy, hyoid arch; lr, lateral rectus EOM; ma, mandibular arch;
nt, neural tube; ov, otic vesicle; t, telencephalon; vr, ventral rectus
EOM.
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