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Fig. 6. The signal from the neural tube is a soluble factor. Internal views
of Paraxis-RMO270 stained, bisected embryo at HH20.
(A,D) Unoperated control sides,
(B,C,E,F) operated sides before (B,E) and after
(C,F) removal of the barrier or filter. Anterior is to the top, dorsal to the
left in A,D; to the right in B,C,E,F. Position of the barrier or filter is
indicated. (A-C) Separation of neural tube and head mesoderm with tantalum
foil, an impermeable barrier. Note that on the control side the abducens nerve
is well fasciculated and innervated the Paraxis-expressing lateral
rectus anlage (A). On the operated side (B,C), the nerve is defasciculated and
did not reach the area that normally would express Paraxis next to
rhombomere 2. (D,E) Separation of neural tube and head mesoderm with a
polycarbonate filter that admits soluble factors. Note that Paraxis
is expressed on the operated side. Moreover, the abducens nerve navigated
around the filter and innervated the Paraxis-expressing lateral
rectus. Scale bar: 200 µm. r, rhombomere; VI, abducens nerve.