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Fig. 8. The signal from the neural tube is not rhombomere 2-specific.
(A) Scheme of neural tube electroporation at HH10. (B-G) Lateral
views of electroporated embryos at HH19; anterior to the top, dorsal to the
left. (B,E) The targeted area revealed by means of eGFP fluorescence.
(C,D,F,G) Paraxis (C,F) or Myf5 (D,G) expression in blue and
staining with the anti-GFP antibody in brown. Same embryo shown in B,C and
E,F, respectively. (B-D) Control electroporation with the pCAß-IRES-eGFP
vector lacking the open reading frame for Hoxb1 allows normal
expression of Paraxis and Myf5. (E-G) Misexpression of
Hoxb1 in the anterior hindbrain transforms the identity of
rhombomeres into that of rhombomere 4 (r1*, r2*).
However, Paraxis and Myf5 expression are not perturbed.
Scale bar: 250 µm. d, diencephalon; hy, hyoid arch; m, midbrain; ma,
mandibular arch; ov, otic vesicle; r, rhombomere; t, telencephalon.
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