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Fig. 1. Live morphology of MZspg and Mspg mutant embryos.
(A,A') Until sphere stage, mutants are indistinguishable
from wild-type embryos. (B,B') Doming and epiboly is
inefficient in MZspg embryos and (C,C') the blastoderm fails to
flatten. (C-D') The shield forms on time, but the blastoderm has
only reached 40% E in MZspg embryos. Shield and germring are thicker
when compared with the wild type. (E-H') Epiboly of the YSL and
EVL (yellow arrows) is uncoupled from epiboly of the blastoderm (red arrows)
in MZspg embryos, which is stalled when the blastoderm covers around
60% of the yolk. (I) The notochord is split in MZspg embryos
(I') and the somites fuse on the opposite site (I'').
(J-N) After 1 day of development. (K,L) MZspg embryos display
severe morphological abnormalities compared with wild type (J) and exhibit
massive cell death (arrow in K indicates the split notochord; arrowhead in K
and L indicates ventrally fused somites). (E''-H'') Mspg
embryos recover completely from their initial epiboly defect until the end of
gastrulation. Expressivity of the Mspg phenotype is variable:
`strong' Mspg embryos (M) are dorsalized (H'',N) whereas `mild'
Mspg embryos are hardly dorsalized.
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