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Fig. 6. Spatial requirement for pou2. Animal views, if not noted
otherwise. (A-M) An asterisk indicates the shield. (F',H,J,L,N) Upper half
with and lower half without phase contrast to show the shield, or expression,
respectively. (A'-M') Bright-field images of injected living
embryos were merged with UV images taken with the same adjustment.
(A''-M'') UV images show EGFP expression, corresponding to
A'-M'. (A-J) pou2 mRNA injection into
ventro-marginal blastomeres of MZspg embryos at early cleavage stages
can restore bmp4 expression. (B') bmp4 expression at
beginning of shield stage in the wild-type embryo. (K-N) pou2
mRNA injection into dorsal or animal blastomeres of MZspg embryos at
early cleavage stages cannot restore bmp4 expression.